8AOQ

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 20a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	56.660, 59.169, 88.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.160 - 2.088
Rwork	0.195
R-free	0.23250
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4v2y
RMSD bond length	0.007
RMSD bond angle	1.463
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.210
High resolution limit [Å]	2.088	2.090
Rmerge	0.064	0.857
Number of reflections	18215	5523
<I/σ(I)>	17.04	2
Completeness [%]	100.0	99.9
Redundancy	7	6.96
CC(1/2)	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.4M Ammonium Phosphate