8ANQ
Crystal structure of the microbial rhodopsin from Sphingomonas paucimobilis (SpaR)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-24 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.975997 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 233.339, 65.027, 124.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.910 - 2.800 |
R-factor | 0.2761 |
Rwork | 0.275 |
R-free | 0.30330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.002 |
RMSD bond angle | 1.082 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.320 | 46.320 | 2.910 |
High resolution limit [Å] | 2.800 | 10.480 | 2.800 |
Rmerge | 0.171 | 0.021 | 1.245 |
Rmeas | 0.218 | 0.028 | 1.592 |
Rpim | 0.133 | 0.018 | 0.980 |
Total number of observations | 104259 | 1900 | 10595 |
Number of reflections | 44606 | 865 | 4702 |
<I/σ(I)> | 4.6 | 20 | 0.7 |
Completeness [%] | 96.4 | 91.9 | 97.3 |
Redundancy | 2.3 | 2.2 | 2.3 |
CC(1/2) | 0.995 | 1.000 | 0.396 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 1.6M Ammonium Phosphate pH 5.2 |