8ANO
Fucosylated mixed-chirality linear peptide FHP8 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 1.3 Angstrom resolution.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-13 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000023 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.805, 62.901, 64.565 |
Unit cell angles | 90.00, 92.87, 90.00 |
Refinement procedure
Resolution | 45.030 - 1.290 |
R-factor | 0.1683 |
Rwork | 0.167 |
R-free | 0.20170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xoc |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.030 | 1.292 |
High resolution limit [Å] | 1.285 | 1.285 |
Rmeas | 0.099 | |
Number of reflections | 207392 | 31662 |
<I/σ(I)> | 8.08 | 0.81 |
Completeness [%] | 98.2 | 92.7 |
Redundancy | 3.22 | |
CC(1/2) | 0.997 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M HEPES pH 7.5, 25% w/v polyethylene glycol 3350 |