8AMH
The Crystal structure of wt apo agroavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID09 |
| Synchrotron site | ESRF |
| Beamline | ID09 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.853, 83.639, 67.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.440 - 1.730 |
| R-factor | 0.19374 |
| Rwork | 0.192 |
| R-free | 0.21836 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | rhizavidin |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.687 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.440 | 1.790 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.061 | 1.090 |
| Number of reflections | 47014 | 4789 |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 5.1 | |
| CC(1/2) | 0.990 | 0.639 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG 3350, 0.1 M MgCl2, 0.1 M Bis-Tris pH 6 |
