8ALM

X-ray structure of human NCS-1 bound to Ric-8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979263
Spacegroup name	P 41 21 2
Unit cell lengths	56.639, 56.639, 134.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.200 - 1.854
R-factor	0.2118
Rwork	0.210
R-free	0.25250
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.136
Data reduction software	AutoProcess (1.0.5)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.201	1.942
High resolution limit [Å]	1.854	1.854
Number of reflections	17666	881
<I/σ(I)>	15.2
Completeness [%]	91.1
Redundancy	8.7
CC(1/2)	0.999	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	30 % PEG 4000, 100 mM sodium acetate pH 4.6