8AHZ
Native VirD of Streptomyces virginiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-09-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.953709 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.310, 84.310, 230.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.780 - 1.700 |
R-factor | 0.1602 |
Rwork | 0.159 |
R-free | 0.17980 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.517 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.620 | 2.100 | 1.810 |
High resolution limit [Å] | 1.700 | 2.000 | 1.760 |
Rmerge | 0.071 | 0.030 | 0.753 |
Rmeas | 0.074 | 0.032 | 0.788 |
Rpim | 0.021 | 0.015 | 0.226 |
Number of reflections | 87602 | 1110 | 5171 |
<I/σ(I)> | 19.5 | ||
Completeness [%] | 99.0 | 99.9 | 85.9 |
Redundancy | 12.6 | 10.2 | 11.6 |
CC(1/2) | 0.997 | 0.999 | 0.810 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 400, 20% PEG 800, 100 mM Tris-HCl |