8AHN

Sin Nombre virus Gn in complex with Fab SNV-42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	88.926, 146.420, 157.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.620 - 1.800
R-factor	0.1911
Rwork	0.190
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 5OPG and PDB ID 5UR8
RMSD bond length	0.008
RMSD bond angle	0.998
Data reduction software	xia2 (3.8.0-g3d57088-dials-3.8)
Data scaling software	xia2 (3.8.0-g3d57088-dials-3.8)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.620	46.640	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.120
Rmeas	0.125	0.056	1.434
Rpim	0.033	0.015	0.379
Number of reflections	95210	5023	4682
<I/σ(I)>	13.4	50.4	1.1
Completeness [%]	100.0	100	99.7
Redundancy	13.8	14	14
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	294	0.2 M tri-ammonium citrate pH 7.0 and 20% w/v polyethylene glycol 3350