8AHB

rsEGFP2 photoswitched to its off-state at room temperature and back-switched to its on-state at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	51.668, 61.759, 70.445
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.440 - 1.790
R-factor	0.1934
Rwork	0.192
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dty
RMSD bond length	0.006
RMSD bond angle	0.919
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP (11.7.03)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	1.858
High resolution limit [Å]	1.790	1.790
Rmerge	0.127
Rpim	0.038
Number of reflections	254080	14673
<I/σ(I)>	10.87	0.45
Completeness [%]	99.4	94.43
Redundancy	11.8	7.3
CC(1/2)	0.999	0.237

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	293	100 mM HEPES buffer, pH 8.1 1.9 M ammonium sulfate