8AHA
rsEGFP2 photoswitched to its off-state at 100K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-03 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.400, 60.755, 71.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.320 - 2.380 |
R-factor | 0.1882 |
Rwork | 0.186 |
R-free | 0.22340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dtx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | autoPROC (1.1.7) |
Data scaling software | autoPROC (1.1.7) |
Phasing software | MOLREP (11.7.03) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.320 | 2.465 |
High resolution limit [Å] | 2.380 | 2.380 |
Rpim | 0.071 | 0.408 |
Number of reflections | 124380 | 12739 |
<I/σ(I)> | 11.44 | 2.23 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13 | 13.5 |
CC(1/2) | 0.996 | 0.714 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 100 mM HEPES buffer, pH 8.1 1.9 M ammonium sulfate |