8AFP

Structure of fibronectin 2 and 3 of L1CAM at 3.0 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	C 2 2 21
Unit cell lengths	55.477, 96.666, 106.578
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.800 - 3.000
R-factor	0.2404
Rwork	0.240
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.236
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.250
High resolution limit [Å]	2.950	2.950
Rmeas	0.160	1.000
Number of reflections	11342	548
<I/σ(I)>	12.1
Completeness [%]	97.3
Redundancy	13.9
CC(1/2)	0.990	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	150 mM sodium citrate pH 5.5, 10.6% PEG 20.000, 10 mM sodium potassium tartrate