8AFK

Structure of iRFP variant C15S/N136R/V256C in complex with phycocyanobilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	I 2 2 2
Unit cell lengths	77.806, 98.708, 156.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.015
R-factor	0.2422
Rwork	0.240
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e04
RMSD bond length	0.008
RMSD bond angle	1.110
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	BUSTER (2.10.4 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.700	2.182
High resolution limit [Å]	2.015	2.015
Rpim	0.030	1.406
Number of reflections	30361	1898
<I/σ(I)>	14.257
Completeness [%]	75.4
Redundancy	13.6
CC(1/2)	0.999	0.283

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10% PEG1000 10% PEG8000