8AFJ
tRNA modifying enzyme MiaE soaked in Na-dithionite in a glovebox and flash-cooled using a miniature-airlock
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-13 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.276, 51.845, 78.183 |
| Unit cell angles | 90.00, 90.63, 90.00 |
Refinement procedure
| Resolution | 40.620 - 1.600 |
| R-factor | 0.201 |
| Rwork | 0.200 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zmb |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.410 | 1.790 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmeas | 0.062 | 0.660 |
| Number of reflections | 37525 | 1808 |
| <I/σ(I)> | 8.5 | 1.8 |
| Completeness [%] | 83.1 | 55.1 |
| Redundancy | 3 | 2.5 |
| CC(1/2) | 0.990 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.5M CaCl2, 25% PEG 6 K and 0.2M Tris buffer at pH 8.5 and 10 mM sodium dithionite |
