8AF9

Structure of a T4SS effector from Brucella sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97242
Spacegroup name	P 61 2 2
Unit cell lengths	133.909, 133.909, 389.511
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.610 - 2.500
R-factor	0.2174
Rwork	0.216
R-free	0.24480
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.690	48.690	2.550
High resolution limit [Å]	2.500	12.250	2.500
Rmerge	0.184	0.107	1.234
Rmeas	0.189	0.112	1.273
Rpim	0.043	0.033	0.310
Total number of observations	1420281	11765	73144
Number of reflections	72439	760	4384
<I/σ(I)>	12.8	20.7	2.6
Completeness [%]	100.0	98.4	100
Redundancy	19.6	15.5	16.7
CC(1/2)	0.993	0.992	0.927

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	25% PEG4000 0.2M CaCl2 100 mM Tris pH 8