8AD9
Crystal structure of ClpC2 C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.582, 40.455, 59.741 |
| Unit cell angles | 90.00, 112.47, 90.00 |
Refinement procedure
| Resolution | 38.670 - 1.430 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.21090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wdc |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.670 | 38.670 | 1.450 |
| High resolution limit [Å] | 1.430 | 7.830 | 1.430 |
| Rmerge | 0.057 | 0.058 | 0.924 |
| Rmeas | 0.062 | 0.065 | 1.003 |
| Rpim | 0.024 | 0.028 | 0.384 |
| Total number of observations | 393155 | 2120 | 18785 |
| Number of reflections | 57672 | 385 | 2843 |
| <I/σ(I)> | 14.9 | 33.8 | 2.1 |
| Completeness [%] | 98.6 | 98.1 | 97.9 |
| Redundancy | 6.8 | 5.5 | 6.6 |
| CC(1/2) | 0.998 | 0.990 | 0.762 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | 100 mM C2H3NaO2 pH 4.5, 30 % (w/v) PEG 8K, 200 mM LiSO4 |
