8ACX
Pathogen effector of Zymoseptoria tritici: Zt-KP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-03 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 109.108, 109.108, 252.755 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.440 - 1.900 |
| R-factor | 0.1715 |
| Rwork | 0.171 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Itasser model |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.889 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 86240 | 2800 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 97.0 | |
| Redundancy | 9.25 | |
| CC(1/2) | 0.999 | 0.381 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | sodium acetate and PEG 300 |
