8AC8

The nucleoprotein complex of Rep protein with DUE ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979310
Spacegroup name	P 2 21 21
Unit cell lengths	47.400, 85.310, 146.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.850 - 1.600
R-factor	0.2129
Rwork	0.212
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rep
RMSD bond length	0.006
RMSD bond angle	0.828
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.850	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	79263	12422
<I/σ(I)>	17.92
Completeness [%]	99.6
Redundancy	12.99
CC(1/2)	0.990	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG 8000, ethylene glycol, HEPES, halogens