8ABQ
Structure of the SNX1-SNX5 complex, Pt derivative
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07158 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 52.690, 51.470, 192.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.470 - 2.810 |
| R-factor | 0.2571 |
| Rwork | 0.252 |
| R-free | 0.30550 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.18-3855-000) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 192.319 | 51.469 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.850 | 2.800 |
| Rmerge | 0.033 | 0.991 | |
| Rmeas | 0.095 | 0.060 | 1.182 |
| Rpim | 0.038 | 0.027 | 0.469 |
| Total number of observations | 155315 | 4905 | 22205 |
| Number of reflections | 24815 | 840 | 3609 |
| <I/σ(I)> | 10.3 | 31 | 1.7 |
| Completeness [%] | 96.2 | 95.6 | 96.1 |
| Redundancy | 6.3 | 5.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 11% PVA, 10% 1-propanol, 100mM HEPES pH 7.5 |
