8AAS

Crystal structure of the Pyrococcus abyssi RPA trimerization core bound to poly-dT20 ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 32 2 1
Unit cell lengths	75.234, 75.234, 215.487
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.260 - 3.200
R-factor	0.2655
Rwork	0.264
R-free	0.29330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Working model of the full apo-RPA
RMSD bond length	0.008
RMSD bond angle	0.850
Data reduction software	XDS (VERSION Feb 5, 2021)
Data scaling software	STARANISO (2.3.77)
Phasing software	MOLREP (11.7.03)
Refinement software	BUSTER (2.10.4 (3-FEB-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.260	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.067	4.189
Rmeas	0.068	4.270
Rpim	0.013	0.816
Number of reflections	10041	204
<I/σ(I)>	27.1	1
Completeness [%]	80.7	21.2
Redundancy	27.2	26.8
CC(1/2)	1.000	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	10% w/v PEG 8K 0.1M imidazole pH 8 0.2 M calcium acetate