8A9I

PI3KC2a core in complex with PITCOIN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	56.157, 133.292, 152.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.490 - 2.870
R-factor	0.2188
Rwork	0.217
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7bi4
RMSD bond length	0.006
RMSD bond angle	1.372
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.490	3.040
High resolution limit [Å]	2.870	2.870
Rmeas	0.164	1.958
Number of reflections	26676	4274
<I/σ(I)>	9.93
Completeness [%]	98.7
Redundancy	4.02
CC(1/2)	0.995	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	0.1MTris, 8-6% PEG3350, 0.2-0.1M MgSO4