8A90
Crystal structure of FrsH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999987 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 117.715, 117.715, 234.261 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.020 - 2.574 |
R-factor | 0.2185 |
Rwork | 0.217 |
R-free | 0.26390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jo0 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.882 |
Data reduction software | XDS (1.20_4444) |
Phasing software | PHENIX (1.20_4444) |
Refinement software | PHENIX (1.20_4444) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.020 | 2.665 |
High resolution limit [Å] | 2.574 | 2.574 |
Rmerge | 0.151 | |
Number of reflections | 87244 | 4482 |
<I/σ(I)> | 7.22 | 1.01 |
Completeness [%] | 93.0 | |
Redundancy | 4.2 | |
CC(1/2) | 0.991 | 0.458 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.10 M HEPES pH 7.8, 10.89% (w/v) PEG 20000, 0.08 M KAc, 1.36% glycerol |