8A5U

Crystal structure of the beta3 subunit extracellular domain of nicotinic acetylcholine receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-10
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	50.998, 66.772, 73.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.450 - 2.400
R-factor	0.2137
Rwork	0.211
R-free	0.28160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uxu
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.450	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.021	0.240
Number of reflections	10319	1020
<I/σ(I)>	16.33
Completeness [%]	99.9	99.51
Redundancy	6
CC(1/2)	1.000	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	10% w/v PEG 6000, 100 mM Bicine pH 9.0