8A59
C-type lectin-like domain (CTLD) and Sushi-like domain of human CD93
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-09 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.712, 112.504, 73.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.500 - 1.920 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AF-Q9NPY3-F1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.260 | 2.020 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.073 | 1.226 |
| Rmeas | 0.076 | 1.278 |
| Rpim | 0.022 | 0.356 |
| Number of reflections | 20515 | 2956 |
| <I/σ(I)> | 12.3 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.3 | 12.7 |
| CC(1/2) | 0.999 | 0.830 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M Ammonium sulfate, 1 M Lithium sulfate and 0.1 M Sodium citrate |
