8A4I

Crystal structure of SALL4 zinc finger cluster 4 with AT-rich DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-27
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.2822
Spacegroup name	P 1
Unit cell lengths	39.026, 66.111, 77.938
Unit cell angles	73.04, 76.43, 76.14

Refinement procedure

Resolution	73.367 - 2.760
R-factor	0.2476
Rwork	0.247
R-free	0.25350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	IDEAL DNA
RMSD bond length	0.006
RMSD bond angle	0.825
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.367	3.337
High resolution limit [Å]	2.760	2.760
Rmerge	0.452	0.906
Rmeas	0.541	1.100
Number of reflections	6179	309
<I/σ(I)>	3.122	1.903
Completeness [%]	0.8	0.2832
Redundancy	3.28	3.07
CC(1/2)	0.890	0.669

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	50 mM MES pH 6.0, 20 % PEG 3350, 60 mM MgCl2