8A4A

Structure of human Rep15 in complex with bovine Rab3C.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977930
Spacegroup name	I 2 2 2
Unit cell lengths	58.290, 108.795, 172.027
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.970 - 2.520
R-factor	0.2093
Rwork	0.207
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rab
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.970	2.610
High resolution limit [Å]	2.520	2.520
Rmerge	0.110	1.365
Rmeas	0.112
Rpim	0.031	0.386
Number of reflections	18879	1827
<I/σ(I)>	18.83	2.05
Completeness [%]	99.2	99.19
Redundancy	13.3	13.2
CC(1/2)	0.999	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.1 M MES pH 6.0 10% w/v PEG 6000