8A44

Plasmodium vivax Duffy binding protein region II bound the DARC ectodomain and monoclonal antibody DB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-14
Detector	DECTRIS EIGER2 S 4M
Wavelength(s)	0.9999
Spacegroup name	P 21 21 21
Unit cell lengths	52.204, 97.949, 287.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.000 - 2.490
R-factor	0.1977
Rwork	0.196
R-free	0.22460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r2s
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.000	2.570
High resolution limit [Å]	2.490	2.490
Rpim	0.070	0.735
Number of reflections	52982	4488
<I/σ(I)>	5.7
Completeness [%]	100.0
Redundancy	1.9
CC(1/2)	0.993	0.463

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	15% w/v jeffamine D-2003 10% v/v ethanol