8A3U
Crystal structure of a chimeric LOV-Histidine kinase SB2F1 (symmetrical variant, trigonal form with short c-axis)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97916 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 138.716, 138.716, 49.346 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.650 - 2.330 |
R-factor | 0.2341 |
Rwork | 0.232 |
R-free | 0.27970 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 0.819 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXD |
Refinement software | PHENIX (1.19rc6_4061) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.650 | 2.510 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.117 | 2.260 |
Rmeas | 0.120 | |
Rpim | 0.027 | 0.549 |
Number of reflections | 13863 | 693 |
<I/σ(I)> | 19.8 | 1.5 |
Completeness [%] | 58.6 | 14.6 |
Redundancy | 20 | |
CC(1/2) | 0.999 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292.15 | 10% PEG8K, 0.1M Citrate, 0.2M NaCl, 0.1M Ammonium Bromide, 1mM ATP, 2mM MgCl2 |