8A2V

Room temperature structure of the ground state of AtPhot2LOV2 in space group P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-02-02
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 43 21 2
Unit cell lengths	40.829, 40.829, 132.814
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.010 - 1.590
Rwork	0.165
R-free	0.19960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qqk
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	1.640
High resolution limit [Å]	1.580	1.590
Rpim	0.071	0.537
Number of reflections	14046	297
<I/σ(I)>	6.3	1.3
Completeness [%]	92.1
Redundancy	8
CC(1/2)	0.990	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	100 mM MES pH 6.0, 4 to 9 % PEG8000, and 50 to 200 mM calcium acetate