8A2G

Crystal structure of Sebokelevirus 2A2 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97957
Spacegroup name	P 21 21 2
Unit cell lengths	64.350, 69.730, 57.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.270 - 1.560
Rwork	0.170
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8a2f
RMSD bond length	0.008
RMSD bond angle	1.486
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.300	1.600
High resolution limit [Å]	1.560	1.560
Rmerge	0.055	0.726
Number of reflections	37407	2713
<I/σ(I)>	26.3	3.9
Completeness [%]	100.0	100
Redundancy	12.9	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350, 0.2M sodium fluoride (according to info sent to us) or 30% PEG4000, 0.005 M magnesium acetate, 0.050 M sodium cacodylate pH 6.5 (thesis info)