8A1G

Structure of the SNX1-SNX5 complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07158
Spacegroup name	P 1 21 1
Unit cell lengths	49.780, 188.470, 51.600
Unit cell angles	90.00, 89.94, 90.00

Refinement procedure

Resolution	49.770 - 2.500
R-factor	0.2492
Rwork	0.243
R-free	0.28450
Structure solution method	MAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	autoSHARP
Refinement software	PHENIX (1.18-38555-000)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.770	49.770	2.650
High resolution limit [Å]	2.500	7.470	2.500
Rmerge	0.137	0.096	0.889
Rmeas	0.151	0.105	0.998
Total number of observations	167629
Number of reflections	32404	1244	4992
<I/σ(I)>	7.15	17.13	1.69
Completeness [%]	98.5	99.3	94.5
Redundancy	5.173	6.369	4.583
CC(1/2)	0.993	0.991	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	11%PVA, 10% 1-propanol, 100mM Hepes pH 7.5