8A16

Human PTPRM domains FN3-4, in spacegroup P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9159
Spacegroup name	P 21 21 21
Unit cell lengths	50.682, 89.638, 128.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.480 - 2.890
R-factor	0.2367
Rwork	0.234
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v5y
RMSD bond length	0.003
RMSD bond angle	0.779
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.290	2.940
High resolution limit [Å]	2.890	2.890
Number of reflections	13719	672
<I/σ(I)>	7
Completeness [%]	100.0
Redundancy	12.4
CC(1/2)	0.991	0.432

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium nitrate and 20% (w/v) PEG 3350