8A0L
Tubulin-CW1-complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 104.630, 156.559, 179.318 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.507 - 1.998 |
| Rwork | 0.188 |
| R-free | 0.22590 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5lxt |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.507 | 47.507 | 2.050 |
| High resolution limit [Å] | 1.998 | 8.940 | 2.000 |
| Rmerge | 0.129 | 0.027 | 4.229 |
| Rmeas | 0.134 | 0.028 | 4.398 |
| Number of reflections | 198578 | 2423 | 14540 |
| <I/σ(I)> | 14.7 | 70.58 | 0.63 |
| Completeness [%] | 100.0 | 99.1 | 99.9 |
| Redundancy | 13.473 | 12.555 | 13.173 |
| CC(1/2) | 0.999 | 1.000 | 0.181 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 11% PEG 4K, 10% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |
