8A0J
Crystal structure of the kinetoplastid kinetochore protein Trypanosoma congolense KKT2 divergent polo-box domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-27 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.700, 57.133, 83.671 |
| Unit cell angles | 90.00, 97.87, 90.00 |
Refinement procedure
| Resolution | 48.240 - 2.200 |
| R-factor | 0.1968 |
| Rwork | 0.194 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alphafold model |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 1.954 |
| High resolution limit [Å] | 1.921 | 1.921 |
| Number of reflections | 33539 | 1736 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 95.8 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 0.1 M HEPES, pH 7.0, 8% w/v Polyvinyl alcohol, 10% v/v 1-Propanol |
