830C
COLLAGENASE-3 (MMP-13) COMPLEXED TO A SULPHONE-BASED HYDROXAMIC ACID
Experimental procedure
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1997-12 |
Detector | ADSC |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.072, 36.098, 96.368 |
Unit cell angles | 90.00, 131.06, 90.00 |
Refinement procedure
Resolution | 7.000 - 1.600 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cgf |
RMSD bond length | 0.010 |
RMSD bond angle | 25.130 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.087 |
Number of reflections | 43128 |
Completeness [%] | 91.0 |
Redundancy | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | 4 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9-12 (mg/ml) | |
2 | 1 | drop | Tris | 20 (mM) | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | 50 (mM) | ||
5 | 1 | drop | inhibitor | 0.3 (mM) | |
6 | 1 | reservoir | PEG4000 | 10-14 (%) | can be replaced by PEG8000 |
7 | 1 | reservoir | ammonium formate | 1.0-1.5 (M) | |
8 | 1 | reservoir | Tris | 100 (mM) |