821P

THREE-DIMENSIONAL STRUCTURES AND PROPERTIES OF A TRANSFORMING AND A NONTRANSFORMING GLYCINE-12 MUTANT OF P21H-RAS

Experimental procedure

Spacegroup name	P 32 2 1
Unit cell lengths	40.200, 40.200, 160.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.500
R-factor	0.22
Rwork	0.220
RMSD bond length	0.015
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.400 *
Rmerge	0.088 *
Total number of observations	102376 *
Number of reflections	25400 *
Completeness [%]	82.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		Schere, A., (1989) J. Mol. Biol., 206, 257. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	3-4 (mg/ml)
2	1	1	Tris-HCl	64 (mM)
3	1	1	dithioerithritol	1 (mM)
4	1	1		10 (mM)
5	1	1	sodium azide	1 (mM)
6	1	2		10 (mM)
7	1	2	PEG400	25 (%(w/v))