7ZYB
BeKdgF with Ca
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 47.374, 47.374, 175.859 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.030 - 1.500 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fpx |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.786 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18_3861) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.030 | 1.554 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 2.167 |
| Rmeas | 0.055 | 2.431 |
| Rpim | 0.014 | 1.069 |
| Number of reflections | 19628 | 1845 |
| <I/σ(I)> | 20.91 | 0.62 |
| Completeness [%] | 98.8 | 95.32 |
| Redundancy | 13.3 | 4.8 |
| CC(1/2) | 0.999 | 0.808 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 80mM MES pH 6.5, 14.4% (w/v) PEG8000, 20% v/v glycerol, 160mM calcium acetate |
