7ZY7
Crystal structure of Chlamydomonas reinhardtii chloroplastic phosphoglycerate kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-04-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980115 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.851, 53.138, 66.406 |
| Unit cell angles | 90.00, 90.70, 90.00 |
Refinement procedure
| Resolution | 42.160 - 1.980 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.22590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Alphafold model |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.160 | 2.046 |
| High resolution limit [Å] | 1.975 | 1.975 |
| Number of reflections | 50948 | 4838 |
| <I/σ(I)> | 9.98 | 1.18 |
| Completeness [%] | 99.3 | 93.38 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.994 | 0.354 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 100 mM MES pH 5, 20% PEG 6000 |
