7ZVU

HUMAN PRMT5:MEP50 Crystal Structure With MTA and Fragment Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9197
Spacegroup name	I 2 2 2
Unit cell lengths	105.152, 139.162, 178.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.750 - 1.950
R-factor	0.2384
Rwork	0.237
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eml
RMSD bond length	0.014
RMSD bond angle	1.803
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.780	2.174
High resolution limit [Å]	1.946	1.946
Number of reflections	60214	3012
<I/σ(I)>	13.8
Completeness [%]	95.0	75.5
Redundancy	12.1
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	14% PEG3350, 100 mM Na citrate pH 5.4, 100mM Carboxylic acids