7ZVR

Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) complexed with zeaxanthin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-12
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	62.688, 66.528, 120.516
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.260 - 2.000
R-factor	0.2375
Rwork	0.235
R-free	0.28390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ztq
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	MOLREP
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.260	60.260	2.050
High resolution limit [Å]	2.000	8.930	2.000
Rmerge	0.125	0.048	1.169
Rmeas	0.133	0.054	1.229
Rpim	0.043	0.024	0.371
Total number of observations	180636	1046	12462
Number of reflections	17115	214	1207
<I/σ(I)>	15	28.2	2
Completeness [%]	98.0	93.9	95.5
Redundancy	10.6	4.9	10.3
CC(1/2)	0.834	0.994	0.657

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS-TRIS propane, pH 7.0