7ZVQ
Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) in the apoform, S206V mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-25 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.985, 65.333, 118.978 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.030 - 2.500 |
R-factor | 0.2007 |
Rwork | 0.198 |
R-free | 0.25360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ztq |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | MOLREP |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 21.030 | 21.030 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.162 | 0.062 | 0.908 |
Rmeas | 0.173 | 0.071 | 0.964 |
Rpim | 0.060 | 0.032 | 0.322 |
Total number of observations | 71106 | 764 | 8353 |
Number of reflections | 8648 | 193 | 946 |
<I/σ(I)> | 8.6 | 13.8 | 1.9 |
Completeness [%] | 99.8 | 92.4 | 99.9 |
Redundancy | 8.2 | 4 | 8.8 |
CC(1/2) | 0.994 | 0.997 | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.5 M Sodium citrate tribasic dihydrate, 0.1 M BISTRIS propane, pH 7.0 |