7ZVQ

Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) in the apoform, S206V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-25
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.54184
Spacegroup name	C 2 2 21
Unit cell lengths	61.985, 65.333, 118.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.030 - 2.500
R-factor	0.2007
Rwork	0.198
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ztq
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	MOLREP
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	21.030	21.030	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.162	0.062	0.908
Rmeas	0.173	0.071	0.964
Rpim	0.060	0.032	0.322
Total number of observations	71106	764	8353
Number of reflections	8648	193	946
<I/σ(I)>	8.6	13.8	1.9
Completeness [%]	99.8	92.4	99.9
Redundancy	8.2	4	8.8
CC(1/2)	0.994	0.997	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.5 M Sodium citrate tribasic dihydrate, 0.1 M BISTRIS propane, pH 7.0