7ZVP
Crystal structure of poplar glutathione transferase U19 in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98010 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.727, 56.727, 182.015 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.140 - 1.610 |
R-factor | 0.2301 |
Rwork | 0.229 |
R-free | 0.24980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7zs3 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 1.640 |
High resolution limit [Å] | 1.610 | 1.610 |
Rmerge | 0.062 | 0.670 |
Number of reflections | 39646 | 1927 |
<I/σ(I)> | 17.7 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10 | 10 |
CC(1/2) | 1.000 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 277 | 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate pH 6.5 and 18 % w/v PEG 8000 |