7ZVJ
Homodimeric structure of LARGE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-21 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.531, 107.377, 100.334 |
| Unit cell angles | 90.00, 120.26, 90.00 |
Refinement procedure
| Resolution | 45.640 - 2.610 |
| R-factor | 0.2159 |
| Rwork | 0.215 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | EMD-14985 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.640 | 2.690 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 52821 | 4425 |
| <I/σ(I)> | 4.5 | 0.4 |
| Completeness [%] | 96.0 | 93 |
| Redundancy | 2.7 | |
| CC(1/2) | 0.983 | 0.149 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0 M Na3PO4 monobasic monohydrate, K3PO4 dibasic / pH 5.0 20% PEG 200 as cryo preservative |
