7ZV6
Crystal Structure of Aichivirus A 2A protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.456, 52.776, 61.470 |
| Unit cell angles | 90.00, 108.63, 90.00 |
Refinement procedure
| Resolution | 49.760 - 2.830 |
| Rwork | 0.240 |
| R-free | 0.25070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zv1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.716 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.760 | 2.980 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.158 | 0.674 |
| Rpim | 0.116 | 0.583 |
| Number of reflections | 11153 | 1247 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 95.4 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Hepes-NaOH pH7.5 20% PEG1500 0.1M CaCl2 |
