7ZV1

Crystal Structure of Aichivirus A 2A protein L64M, L109M mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	52.569, 77.445, 98.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.670 - 1.740
Rwork	0.198
R-free	0.21810
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.329
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.670	1.770
High resolution limit [Å]	1.740	1.740
Rmerge	0.056	1.093
Rpim	0.044	0.840
Number of reflections	41619	2258
<I/σ(I)>	14.8
Completeness [%]	99.0
Redundancy	4
CC(1/2)	0.999	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M MES pH6.75 30% PEG 30000 10mM TCEP