7ZV0
Crystal structure of human STING in complex with 3',3'-c-(2'F,2'dAMP-2'F,2'dAMP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-10 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.541870 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 110.916, 110.916, 35.818 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.080 - 2.310 |
R-factor | 0.2083 |
Rwork | 0.206 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ksy |
RMSD bond length | 0.002 |
RMSD bond angle | 0.500 |
Data reduction software | XDS (xdsgui2) |
Data scaling software | XDS (xdsgui2) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.080 | 2.393 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.153 | 0.816 |
Rmeas | 0.161 | 0.873 |
Rpim | 0.052 | 0.304 |
Number of reflections | 10263 | 983 |
<I/σ(I)> | 14.08 | 2.34 |
Completeness [%] | 99.6 | 97.04 |
Redundancy | 9.5 | 7.7 |
CC(1/2) | 0.997 | 0.801 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Lithium acetate, 20% (w/v) PEG 3350 |