7ZUY
HUMAN PRMT5:MEP50 Crystal Structure With MTA and Fragment Bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-03 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9197 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.262, 139.037, 178.421 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 109.670 - 2.000 |
R-factor | 0.2245 |
Rwork | 0.222 |
R-free | 0.26980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eml |
RMSD bond length | 0.007 |
RMSD bond angle | 1.487 |
Data reduction software | XDS (Feb 5, 2021 (BUILT 20210323)) |
Data scaling software | Aimless (0.7.7) |
Phasing software | REFMAC (5.8.0267) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.670 | 2.230 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 48889 | 2444 |
<I/σ(I)> | 12.8 | |
Completeness [%] | 93.7 | 77.7 |
Redundancy | 11.8 | |
CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 13 % PEG3350, 100 mM Na citrate, pH 5.4, 4% Tacsimate pH 7.0 |