7ZUT
Penicillium expansum chimera loop1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-10-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 44.931, 44.931, 42.829 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.910 - 1.100 |
| R-factor | 0.127 |
| Rwork | 0.126 |
| R-free | 0.14590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ztf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.421 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.049 | 0.110 |
| Rpim | 0.012 | 0.035 |
| Number of reflections | 20337 | 2596 |
| <I/σ(I)> | 40.8 | |
| Completeness [%] | 98.0 | |
| Redundancy | 17.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 3.5M ammonium sulphate, 1% MPD, 0.1M Mes pH 6.5 |
