7ZUQ

HUMAN PRMT5:MEP50 Crystal Structure With MTA and Fragment Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9198
Spacegroup name	I 2 2 2
Unit cell lengths	102.862, 138.228, 178.365
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.260 - 2.480
R-factor	0.2291
Rwork	0.227
R-free	0.27940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eml
RMSD bond length	0.005
RMSD bond angle	1.347
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210205))
Data scaling software	Aimless (0.7.4)
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.260	2.840
High resolution limit [Å]	2.480	2.480
Number of reflections	23243	1163
<I/σ(I)>	10
Completeness [%]	92.4	77.6
Redundancy	23
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	12.5% PEG3350, 100 mM Na citrate pH 5.4, 120 mM Ethylene glycols