7ZUP

Human PRMT5:MEP50 structure with Fragment (Example 18) and MTA Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9197
Spacegroup name	I 2 2 2
Unit cell lengths	104.282, 138.654, 178.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.590 - 2.010
R-factor	0.2214
Rwork	0.219
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eml
RMSD bond length	0.007
RMSD bond angle	1.520
Data reduction software	XDS (Feb 5, 2021 (BUILT 20210323))
Data scaling software	Aimless (0.7.7)
Phasing software	REFMAC (5.8.0267)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.590	2.260
High resolution limit [Å]	1.970	1.970
Number of reflections	39692	1985
<I/σ(I)>	10.7
Completeness [%]	93.3	90.9
Redundancy	11.9
CC(1/2)	0.990	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	15% PEG3350, 100 mM Na citrate, pH 5.4, 100mM Carboxylic acids