7ZUO

Crystal Structure of Ljungan virus 4 2A2 protein, P212121 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0,97818
Spacegroup name	P 21 21 21
Unit cell lengths	83.179, 100.314, 120.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.775 - 2.070
Rwork	0.172
R-free	0.20460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zua
RMSD bond length	0.007
RMSD bond angle	1.331
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0349)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.775	2.120
High resolution limit [Å]	2.070	2.070
Rmerge	0.081	0.733
Rpim	0.053	0.638
Number of reflections	61727	4511
<I/σ(I)>	11.6
Completeness [%]	99.7
Redundancy	3.4
CC(1/2)	0.998	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.1M Tris-HCl pH8.0 8% PEG 6000 0.15M NaCl