7ZUA

Crystal Structure of Ljungan virus 4 2A2 protein I222 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00003
Spacegroup name	I 2 2 2
Unit cell lengths	58.513, 59.562, 72.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.106 - 1.680
Rwork	0.209
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ztw
RMSD bond length	0.007
RMSD bond angle	1.384
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.110	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.041	0.638
Rpim	0.037	0.467
Number of reflections	14774	760
<I/σ(I)>	11.4
Completeness [%]	99.6
Redundancy	3.3
CC(1/2)	0.999	0.642

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.25	277	0.1M Na acetate pH 5.25 10% PEG 6000